品牌:Emfret
貨號:P150-1
品名:Emfret FITC-labeled Rabbit Anti-von Willebrand factor (vWf)
規(guī)格:1.5 ml / 300 tests
同種型:Rabbit polyclonal IgG
包含:FITC-labeled immunoglobulin in 20 mM Tris buffer with 137 mM NaCl, 0.5%
BSA and 0.09% (w/v) sodium azide
特異性:
The polyclonal antibody reacts with murine and human von Willebrand factor (vWf), a
multimeric plasma protein that is stored in α-granules of platelets and Weibel-Palade bodies of
endothelial cells1
. The major receptors for vWf are GPIbα (CD42b) and activated integrin αIIbβ3
(CD41/61). GPIbα-vWf interaction can be induced by the snake venom protein botrocetin2
.
用途:
The antibody preparation is optimized for flow cytometric applications: Use 5 μl to stain ~106
platelets or ~0.5x106 cells in a recommended volume of 25 μl. Incubate for 15 minutes at room
temperature, stop reaction by addition of 400 μl PBS and analyse samples within 30 minutes.
For immunofluorescent staining of acetone-fixed frozen sections, the appropriate dilution must be
determined individually.
制備和儲存:
The immunoglobulin was conjugated with FITC under optimum conditions.
The solution is free of unbound FITC. Store product undiluted at 4°C and avoid prolonged exposure to
light. Stable for one year from date of shipment. Do not freeze.
注:僅供研究使用,不用于診斷或治療用途。