品牌：Emfret
貨號：M042-0
品名：Emfret Purified Rat Anti-Mouse GPIba (CD42b)
規(guī)格：0.5 mg/ml
克隆：Xia.G7 / Rat (Wistar) IgG1
包含：0.5 mg purified immunoglobulin in PBS and 0.09% (w/v) sodium azide
特異性：
The Xia.G7 antibody reacts with mouse GPIba (CD42b), a platelet/megakaryocytespecific 150 kDa polypeptide that is disulfide-linked with GPIbb (24 kDa) in the membrane. GPIb is
part of the GPIb-V-IX complex, the platelet receptor for von Willebrand factor (vWf)1,2. Xia.G7 binds to
the 130 kDa extracellular domain of GPIba
3
(glycocalicin), which is proteolytically released during
platelet activation. Xia.G7 recognizes the membrane-bound and the soluble form of GPIba
3
.
用途：
This preparation can be used for immunoprecipitation, immunohistochemical analysis of
acetone-fixed frozen sections, and immunofluorescent staining (1-3 μg/106
platelets or cells). Xia.G7
recognizes mouse GPIba in a Western blot analysis under non-reducing condtions3
.
For varying applications, appropriate dilutions must be determined individually.
制備和儲存：
The antibody was purified from hybridoma cell culture supernatant by
Protein G-Sepharose chromatography. Stable for six months from date of shipment when stored at
4°C. Aliquots can be stored at -20°C for at least one year. Avoid repeated freezing and thawing.
注意：
Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations
containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may
be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free
buffer.
注：僅供研究使用，不用于診斷或治療用途。
