ELISA of IgG from BalB/C and C57BL6 shows RM223 reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM223 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG2c. The plate was coated with different amounts of mouse IgG2c. A serial dilution of RM223 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Western blot of nonreduced(-) and reduced(+) mouse IgG2c, using 0.5ug/mL of RevMAb clone RM223. This antibody reacts to nonreduced IgG2c
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