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Anti-Acetyl-Histone H2B (Lys23) Rabbit Monoclonal Antibody, Histone H2B (Acetyl Lys23), Clone RM260
品牌:RevMAb
貨號:31-1141-00
規(guī)格:100 ug
貨期:電詢
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Product Details
Product Name Anti-Acetyl-Histone H2B (Lys23) Rabbit Monoclonal Antibody [RM260]
Product Description Rabbit monoclonal to Acetyl-Histone H2B (Lys23)
Catalog No. 31-1141-00
Clone Name RM260
Product Data Sheet Anti-Acetyl-Histone H2B (Lys23) Rabbit Monoclonal Antibody Data Sheet RM260
Specificity RM260 reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with other acetylated Lysines in histones.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys23).
Application Western Blot (WB)
ELISA
Multiplex
Immunocytochemistry
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL – 2 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.

Keywords
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Product Images

RM260 specifically reacts to Histone H2B acetylated
at Lysine 23 (K23ac). No cross reactivity with acetyl-
ated Lysine 5 (K5ac), Lysine 11 (K11ac), Lysine 12
(K12ac), Lysine 15 (K15ac), Lysine 20 (K20ac), or
non-mordified Lysine 23 in histone H2B.

Western Blot of (1) acid extracts of HeLa cells treated
with sodium butyrate, (2) acid extracts of HeLa cells
untreated, and (3) recombinant Histone H2B, using
Acetyl-Histone H2B (Lys23) rabbit monoclonal antibody
(RM260) at 0.5 ug/mL.

Immunocytochemistry

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Acetyl-Histone H2B (Lys23)
rabbit monoclonal antibody (RM260) (red). Actin
filaments have been labeled with fluorescein
phalloidin (green).


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